ADRENERGIC MECHANISM IN <I>TETRAHYMENA</I> III CYCLIC ADENOSINE 3', 5'-MONOPHOSPHATE AND CELL PROLIFERATION

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  • KARIYA Kimio
    Department of Pharmacology, Faculty of Pharmacy, Kobe-Gakuin University
  • SAITO Katsuhisa
    New Product Planning and Development Division, Pharmaceutical Affairs, Takeda Chemical Industries LTD.
  • IWATA Heitaroh
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University

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Other Title
  • Adrenergic mechanism in Tetrahymena-3-Cyclic adenosine 3',5'-monophosphate and cell proliferation
  • ADRENERGIC MECHANISM IN TETRAHYMENA III CYCLIC ADENOSINE 3', 5'-MONOPHOSPHATE AND CELL PROLIFERATION

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The presence of cyclic adenosine 3', 5'-monophosphate (cAMP) in the ciliated protozoan, Tetrahymena pyrifurmis W, was demonstrated by thin-layer chromatography (TLC). The content of cAMP in the cell increased abruptly in the early exponential phase, and then diminished with further culture. When cells in the exponential or stationary growth phase were cultured in a fresh medium, growth was inhibited by addition of dibutyryl cAMP (Bt2-cAMP) or methylxanthines. In synchronized cultures of Tetrahymena, Bt2-cAMP inhibited protein and RNA syntheses in the G1 phase and DNA synthesis in the S phase. These results indicate that cAMP regulates the growth of the protozoan in the G1 phase.

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