Development of Contact Photosensitization Test in Guinea Pigs (Adjuvant-Strip Method)

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  • モルモットによる光接触感作性試験法(Adjuvant-Strip法)
  • モルモット ニヨル ヒカリ セッショク カンサセイ シケンホウ Adjuvan
  • モルモットによる光接触感作性試験法（Ａｄｊｕｖａｎｔ‐Ｓｔｒｉｐ法）

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Assay of contact photosensitivity in guinea pigs was developed to detect weak photoallergens (adjuvant-strip method). This test consisted of; (1) using Freund’s complete adjuvant to stimulate the immune system, (2) stripping the nuchal skin with adhesive tape before application so that test material and ultraviolet ray would penetrate into a deeper layer of the skin and (3) irradiating ultraviolet ray without UV-B (280∼320 nm) on induction and challenge stages. With this test, both 6-methyl coumarin (6-MC) and bithionol (TBP) were found to be photosensitizers. The maximum absorption spectrum of 6-MC was 276 nm and that of TBP was 308nm. It should be noted that contact photosensitization is induced with UV-A (320∼400 nm) regardless of the maximum absorption of photoallergens.

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