Microscopic Imaging Measurement of Stomatal Reaction under Environmental Condition Change

  • NAMBA Kazuhiko
    The Japanese Society of Agricultural Machinery Faculty of Agriculture, Okayama University
  • KONDO Naoshi
    The Japanese Society of Agricultural Machinery Department of Technology Development, Ishii Industry Co., Ltd.
  • MONTA Mitsuji
    The Japanese Society of Agricultural Machinery Faculty of Agriculture, Okayama University
  • SASAO Akira
    The Japanese Society of Agricultural Machinery Faculty of Agriculture, Tokyo University of Agriculture and Technology

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Other Title
  • 顕微鏡画像による植物気孔の環境応答の計測
  • ケンビキョウ ガゾウ ニ ヨル ショクブツ キコウ ノ カンキョウ オウトウ ノ ケイソク

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Abstract

The purpose of this study was to create a mathematical model for a responding process of a plant stoma to a given environmental condition aiming at plant growth control. A real-time measurement system was developed to observe a living plant stoma continuously for a long period in a growth chamber, using a microscope equipped with a color TV camera. Sixteen treatments were investigated by changing PPFD (Photosynthetic Photon Flux Density), CO2 concentration, temperature and humidity, under the condition that the light was turned on and off every 6 hours. From the experimental results, it was observed that the stoma response was drastically changed by the environmental conditions and that the transient response with the 1st-order lag element could be adopted for the stoma response in cases of a higher PPFD and higher temperature. It was also observed that the stoma widely opened at a lower CO2 concentration.

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