Photochemical ODN Manipulation Based on Reversible DNA Photoligation Mediated by Modified Photoresponsive Base

DOI Web Site Web Site 参考文献28件
  • Ogino Masayuki
    The School of Materials Science, Japan Advanced Institute of Science and Technology
  • Ogasawa Shinji
    The School of Materials Science, Japan Advanced Institute of Science and Technology
  • Ami Takehiro
    The School of Materials Science, Japan Advanced Institute of Science and Technology
  • Yoshimura Yoshinaga
    PRESTO, Japan Science and Technology Agency
  • Fujimoto Kenzo
    The School of Materials Science, Japan Advanced Institute of Science and Technology PRESTO, Japan Science and Technology Agency

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We describe an efficient template-directed photoligation of oligodeoxynucleotides (ODNs) using modified photoresponsive base. An efficient photoligation was produced by photoirradiation of an ODN containing α-5-cyanovinyldeoxyuridine (αCU) at the 3' end with an ODN containing thymine at the 5' end in the presence of a template ODN. This photoligation method is a new, efficient way to synthesize branched ODNs

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