Intermolecular Interaction of Cyclodextrin Derivatives Immobilized onto the Self-Assembled Phospholipid Layer Fabricated by Plasma-Assisted Method

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  • Kondo Shin-ichi
    Laboratory of Pharmaceutical Physical Chemistry, Gifu Pharmaceutical University
  • Suzuki Masako
    Laboratory of Pharmaceutical Physical Chemistry, Gifu Pharmaceutical University
  • Sasai Yasushi
    Laboratory of Pharmaceutical Physical Chemistry, Gifu Pharmaceutical University
  • Yamauchi Yukinori
    Department of Pharmaceutical Physical Chemistry, College of Pharmaceutical Sciences, Matsuyama University,
  • Kuzuya Masayuki
    Department of Health and Welfare, Faculty of Human Welfare, Chubu Gakuin University,

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Inclusion complex between Per-6-ABCD-Cy3 (per-6-amino-β-cyclodextrin labeled with Cy3) and Per-6-ABCD-Cy5 immobilized onto the self-assembled phospholipid layer was formed, so that FRET was observed. The inclusion complex was decomposed by the addition of PEG. It was suggested that PEG could be detected with the present film and that the amount of PEG might depend on the decrease of fluorescence intensity. We are now actively elaborating the high sensitive detection of PEG with the present LDPE-StA-PC-SA film immobilizing Per-6-ABCD-Cy3 (self-assembled PC layer incorporating StA) and Per-6-ABCD-Cy5.

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