Evaluation of selected transgenic papaya (Carica papaya L.) lines for inheritance of resistance to papaya ringspot virus and horticultural traits

DOI Web Site 参考文献36件
  • Retuta Abigail May O.
    Philippine Council for Agriculture, Aquatic, Forestry and Natural Resources Research and Development (PCAARRD) Philippine Council for Agriculture, Aquatic, Forestry and Natural Resources Research and Development (PCAARRD)
  • Magdalita Pablito M.
    Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (UPLB) Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (UPLB)
  • Aspuria Evalour T.
    Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (UPLB) Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (UPLB)
  • Espino Rene Rafael C.
    Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (UPLB) Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (UPLB)

書誌事項

タイトル別名
  • Evaluation of selected transgenic papaya (<i>Carica papaya</i> L.) lines for inheritance of resistance to papaya ringspot virus and horticultural traits

この論文をさがす

抄録

Three selected transgenic papaya lines 124-3, 132-2 and 142-3 across the T1 and T2 generations were preliminarily evaluated under Biological Containment Level 2 (BL2) greenhouse approved by the National Committee on Biosafety of the Philippines (NCBP) to determine the partial stability of expression of resistance to papaya ringspot virus (PRSV) and selected phenotypic traits. The PRSV reaction of the three transgenic lines using mechanical inoculation test and enzyme-linked immunosorbent assay was assessed. The qualitative and quantitative horticultural traits at flowering and ripe fruit stages of the transgenic lines were also evaluated. Based from preliminary results, the percentages of resistant progenies to PRSV in the T1 generation were 83% for 124-3, 74% for 132-2, and 70% for 142-3. However, in the same generation, no difference between the transgenic and non-transgenic were observed for stem, leaf and petiole color, fruit shape, peel, flesh and seed color, stem diameter, number of days to first flower, and fruit edible portion, indicating that the transgenic is seemingly stable. The percentage of resistant progenies to PRSV in the T1 generation decreased in the T2 generation, indicating that the inherited resistance of the three transgenic lines was unstable, and a continuous evaluation of the transgene could be done in later generations. In contrast, the light green (RHS 141B) stem color, dark green (RHS 141A) leaves and petioles and creamy white flowers (RHS 155A) were stably expressed in both the T1 and T2 generations. The plant height for lines 124-3 and 142-3, internode length for line 142-3 and number of nodes to first flower for lines 132-2 and 142-3 were also stable in both T1 and T2 generations. In addition, the pyriform fruit shape for hermaphrodite sex form and rounded fruit shape for the female sex form, light yellow orange (RHS 17A) peel color, bright yellow orange (RHS 23A) flesh color and black (RHS 202A) seeds of the three transgenic lines were stably expressed in both generations. The fruit weight, total soluble solids and fruit edible portion for lines 124-3 and 142-3 was stably expressed in both generations.

収録刊行物

参考文献 (36)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ