Isolation of polyphenol oxidase genes from Portulaca oleracea and evaluation of their ability to metabolize endocrine-disrupting chemicals
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- Kaneda Hirokazu
- Graduate School of Pharmaceutical Sciences, Osaka University Graduate School of Pharmaceutical Sciences, Osaka University
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- Matsui Takeshi
- Graduate School of Biological Sciences, Nara Institute of Science and Technology Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Tomiyasu Ryosuke
- Graduate School of Pharmaceutical Sciences, Osaka University Graduate School of Pharmaceutical Sciences, Osaka University
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- Kuroda Yukako
- Graduate School of Pharmaceutical Sciences, Osaka University Graduate School of Pharmaceutical Sciences, Osaka University
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- Higashimoto Yuka
- Graduate School of Pharmaceutical Sciences, Osaka University Graduate School of Pharmaceutical Sciences, Osaka University
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- Oda Tomoka
- Graduate School of Pharmaceutical Sciences, Osaka University Graduate School of Pharmaceutical Sciences, Osaka University
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- Miyasaka Hitoshi
- Environmental Research Center, The Kansai Electric Power Co., Inc. Environmental Research Center, The Kansai Electric Power Co., Inc.
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- Okuhata Hiroshi
- Environmental Research Center, The Kansai Electric Power Co., Inc. Environmental Research Center, The Kansai Electric Power Co., Inc.
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- Tanaka Satoshi
- Environmental Research Center, The Kansai Electric Power Co., Inc. Environmental Research Center, The Kansai Electric Power Co., Inc.
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- Harada Kazuo
- Graduate School of Pharmaceutical Sciences, Osaka University Graduate School of Pharmaceutical Sciences, Osaka University
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- Matsuura Hideyuki
- Graduate School of Pharmaceutical Sciences, Osaka University Graduate School of Pharmaceutical Sciences, Osaka University
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- Nakayama Hideki
- Graduate School of Fisheries Science and Environmental Studies, Nagasaki University Graduate School of Fisheries Science and Environmental Studies, Nagasaki University
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- Kato Ko
- Graduate School of Biological Sciences, Nara Institute of Science and Technology Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Hirata Kazumasa
- Graduate School of Pharmaceutical Sciences, Osaka University Graduate School of Pharmaceutical Sciences, Osaka University
書誌事項
- タイトル別名
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- Isolation of polyphenol oxidase genes from <i>Portulaca oleracea</i> and evaluation of their ability to metabolize endocrine-disrupting chemicals
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Portulaca (Portulaca oleracea cv.), a garden plant, efficiently removes endocrine-disrupting chemicals (EDCs) including bisphenol A (BPA) from hydroponic solution. We hypothesized that polyphenol oxidase (PPO) was involved in the initial steps of detoxifying EDCs in portulaca roots. In order to elucidate the molecular basis of portulaca’s ability to metabolize EDCs, we first isolated five PPO genes (PoPPO1–5) that were expressed mainly in portulaca roots. Among these genes, PoPPO2, PoPPO4 and PoPPO5 were introduced into cultured tobacco cells and expressed as active forms. We found that crude extracts from the cells expressing PoPPO2, PoPPO5, and to a lesser extent PoPPO4, could metabolize BPA. In addition, we found that the BPA metabolites from crude extracts of cells expressing PoPPO2, PoPPO4 and PoPPO5 were identical to those of portulaca. Moreover, PoPPO2 and PoPPO5 also caused hydroxylation of octylphenol, nonylphenol and 17β-estradiol. Therefore, these results strongly suggest that PoPPOs significantly contribute to the superior ability of portulaca to metabolize EDCs.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 29 (4), 351-357, 2012
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679303481984
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- NII論文ID
- 10031122159
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BC38XhslSnsbzJ
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 023997309
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可