Microscale thioacidolysis method for the rapid analysis of β-<i>O</i>-4 substructures in lignin
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- Yamamura Masaomi
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Hattori Takefumi
- Institute of Socio-Arts and Sciences, The University of Tokushima
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- Suzuki Shiro
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Shibata Daisuke
- Kazusa DNA Research Institute
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- Umezawa Toshiaki
- Research Institute for Sustainable Humanosphere, Kyoto University Institute of Sustainability Science, Kyoto University
Bibliographic Information
- Other Title
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- Microscale thioacidolysis method for the rapid analysis of β-O-4 substructures in lignin
- Microscale thioacidolysis method for the rapid analysis of ^|^#946;-O-4 substructures in lignin
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Abstract
Thioacidolysis is a method to detect the β-O-4 substructures of lignin, and has been employed as a diagnostic test for the presence of lignin. However, the conventional thioacidolysis protocol is low-throughput and is a bottleneck in the characterization of lignins in a large number of samples such as transgenic lines. Recently, a rapid analysis protocol for thioacidolysis was reported. In this study, we modified mainly the work-up process. Our microscale protocol showed higher yields of thioacidolysis products than the conventional protocol.
Journal
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- Plant Biotechnology
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Plant Biotechnology 29 (4), 419-423, 2012
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390282679303526400
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- NII Article ID
- 10031122169
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- NII Book ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BC38XhslSnsb3I
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 023997482
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed