Induction of Melon Shoot Primordia and Maintenance of Their High Regeneration Potential.

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  • メロンの苗条原基誘導と植物再生能の維持
  • メロン ノ ビョウジョウ ゲンキ ユウドウ ト ショクブツ サイセイノウ ノ

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Abstract

Melon shoot primordia were induced from shoot apex with 2-3 leaf primordia by slanting rotary culture (2rpm) under conditions of continuous light (aprox. 3000 lux). Shoot primordia were induced most effectively on the MS liquid media containing 0.5-4.0mg/l 6-benzyladenine (BA) and 0.01mg/l naphtharenacetic acid (NAA). They were easily subcultured in the MS liquid medium with 1mg/l BA under the same conditions as the initial culture at an interval of 2-3 weeks. Shoot primordia easily developed shoots on the MS gelan gum media containing 0.1-0.4mg/l BA and regenerated plantlets after transferring to the MS hormone free medium. Regeneration potential of subcultured shoot primordia was maintained at a high level, aprox. 80-96% on average after one-year. Melon shoot primordia are available in genetic transformation, or micropropagation, etcetera because of their easiness to subculture, and stable regeneration ability.

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