High-throughput determination of thioglycolic acid lignin from rice

DOI Web Site 被引用文献46件 参考文献21件
  • Suzuki Shiro
    Institute of Sustainability Science, Kyoto University
  • Suzuki Yoshie
    Research Institute of Sustainable Humanosphere, Kyoto University
  • Yamamoto Naoki
    Research Institute of Sustainable Humanosphere, Kyoto University
  • Hattori Takefumi
    Research Institute of Sustainable Humanosphere, Kyoto University
  • Sakamoto Masahiro
    Graduate School of Agriculture, Kyoto University
  • Umezawa Toshiaki
    Institute of Sustainability Science, Kyoto University Research Institute of Sustainable Humanosphere, Kyoto University

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For research and development of plants suitable for forage and biofuel production, the development of a high-throughput system of lignin determination, especially for herbaceous species, is important. However, currently available methods for lignin determination are not suitable for high-throughput analysis of herbaceous samples. In this paper, we describe a straightforward, high-throughput method for lignin determination as thioglycolic acid lignin from rice straw using disposable plastic microtubes, TissueLyser, and a UV microplate reader. We successfully plotted calibration curves derived from the readings of a spectrophotometer and a microplate reader using thioglycolic acid lignin prepared from bamboo milled wood lignin as a lignin standard. Based on the calibration curve, we could determine the lignin content in various organs from dried rice straw and rice seedlings. We confirmed that 20 mg of dried material from rice plants is adequate for stable determination of the lignin content. Using this method, 100 independent samples from rice straw can be analyzed in three days per person.

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