Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (<i>Brassica oleracea</i> subsp. <i>capitata</i>) “KY Cross”

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  • Ravanfar Seyed Ali
    Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia Institute of Tropical Agriculture, Universiti Putra Malaysia
  • Salim Shahida
    Institute of Tropical Agriculture, Universiti Putra Malaysia
  • Aziz Maheran Abd
    Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia Institute of Tropical Agriculture, Universiti Putra Malaysia
  • Abdullah Siti Nor Akmar
    Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia Institute of Tropical Agriculture, Universiti Putra Malaysia
  • Rashid Azmi Abdul
    Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia

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Other Title
  • Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) "KY Cross" Seyed
  • Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) ^|^#8220;KY Cross^|^#8221;

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Description

In this study, the effects of phenyl-urea (Thidiazuron) and adenine (6-benzylaminopurine) and 6- y,y,dimethylally-amino purine type cytokinins alone or in combination with indole-3-butyric acid on shoot regeneration from hypocotyl and cotyledonary explants of Brassica oleracea ssp. capitata “KY Cross” were investigated. For hypocotyl explants, medium containing 2.27 µM Thidiazuron showed the highest mean number (18.15) of separable shoots per explant with 80% shoot formation. In the case of cotyledonary explants, the highest mean number of shoots (3.03) was obtained on medium containing 12.30 µM 6-y,y,dimethylally-amino purine with a percentage of 56.67% shoot formation. Plantlets were successfully acclimatized with 70% survival in potting medium consisting of coconut husk+vermicompost (7 : 1 v/v). The regeneration system developed herewith will be a valuable tool for genetic improvement of cabbage “KY Cross”.

Journal

  • Plant Biotechnology

    Plant Biotechnology 31 (3), 275-280, 2014

    Japanese Society for Plant Biotechnology

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