Molecular characterization of the flavonoid biosynthesis of Verbena hybrida and the functional analysis of verbena and Clitoria ternatea F3'5'H genes in transgenic verbena
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- Togami Junichi
- Institute for Advanced Technology, Suntory, Ltd.
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- Tamura Mihoko
- Institute for Advanced Technology, Suntory, Ltd.
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- Ishiguro Kanako
- Institute for Advanced Technology, Suntory, Ltd.
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- Hirose Chika
- Institute for Advanced Technology, Suntory, Ltd.
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- Okuhara Hiroaki
- Institute for Advanced Technology, Suntory, Ltd.
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- Ueyama Yukiko
- Institute for Advanced Technology, Suntory, Ltd.
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- Nakamura Noriko
- Institute for Advanced Technology, Suntory, Ltd.
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- Yonekura-Sakakibara Keiko
- Institute for Advanced Technology, Suntory, Ltd.
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- Fukuchi-Mizutani Masako
- Institute for Advanced Technology, Suntory, Ltd.
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- Suzuki Ken-ichi
- Institute for Advanced Technology, Suntory, Ltd.
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- Fukui Yuko
- Institute for Advanced Technology, Suntory, Ltd.
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- Kusumi Takaaki
- Institute for Advanced Technology, Suntory, Ltd.
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- Tanaka Yoshikazu
- Institute for Advanced Technology, Suntory, Ltd.
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抄録
Homologues of the flavonoid 3′,5′-hydroxylase (F3′5′H) gene, a key gene determining flower color, were obtained from a Verbena hybrida (verbena) cultivar Temari Violet, verbena cultivar Tapien Pink, and Clitoria ternatea (butterfly pea). The expression of the Temari and butterfly pea homologues in yeast confirmed that they encoded F3′5′H. The two genes under the control of an enhanced cauliflower mosaic virus 35S promoter were introduced into verbena Temari Sakura. Some of the transgenic verbena plants had elevated delphinidin contents and flower color altered toward violet. Interestingly, the butterfly pea F3′5′H gene yielded more delphinidin and gave clearer flower color change than the verbena Temari gene in the transgenic verbena plants. The results indicate that the choice of the gene source should be considered to obtain strong phenotypic changes, even if the genes encode the same enzymatic activity. We also cloned some flavonoid biosynthetic genes from verbenas. The potential usefulness of verbena in the phytomonitoring of environmental pollutants is also discussed.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 23 (1), 5-11, 2006
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679303956992
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- NII論文ID
- 10021907652
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD28XktFaqsrs%3D
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 7857032
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可