Occurrence of Two Forms of .GAMMA.-GIutamyltransferases in Radish Plant
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- NAKANO Yoshihiro
- Plant Nutrition Laboratory, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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- OKAWA Satoshi
- Plant Nutrition Laboratory, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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- YAMAUCHI Takayoshi
- Plant Nutrition Laboratory, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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- KOIZUMI Yukio
- Plant Nutrition Laboratory, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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- SEKIYA Jiro
- Plant Nutrition Laboratory, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Bibliographic Information
- Other Title
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- Occurrence of Two Forms of γ-Glutamyltransferases in Radish Plant
- Occurrence of Two Forms of ガンマ Glutamyltransferases in Radish Plant
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Abstract
In radish (Raphanus sativus L.) cotyledons, γ-glutamyltransferase (GGT, EC 2.3.2.2) activity was detected in both fractions extracted with grinding buffer alone (low ionic strength buffer) and the same buffer supplemented with 1 M NaCl (high ionic strength buffer). The former was referred to as soluble GGT and the latter, as bound GGT. Bound GGT was not solubilized by 1% Triton X-100. The activities of both soluble and bound GGTs were inhibited by acivicin, an inhibitor of mammalian and Escherichia coli GGTs. The activity of GGT extracted with the high ionic strength buffer disappeared when protoplasts were prepared, indicating the localization of bound GGT to cell walls. These results strongly indicate the occurrence of two forms of GGTs, soluble and bound ones. The two forms of GGTs were also found in Arabidopsis thaliana, cabbage, pea, soybean, maize and rice among the eight plants tested, in addition to radish.
Journal
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- Plant Biotechnology
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Plant Biotechnology 21 (3), 243-246, 2004
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390282679304040320
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- NII Article ID
- 10013522948
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- NII Book ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD2cXptVKhuro%3D
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 7066761
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed