Tobacco RETINOBLASTOMA-RELATED protein is phosphorylated by different types of cyclin-dependent kinases during the cell cycle
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- Kawamura Kazue
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Kato Ko
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Shinmyo Atsuhiko
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Sekine Masami
- Department of Bioproduction Science, Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University
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Description
Plants contain a regulatory pathway similar to that involving retinoblastoma (RB) protein in animals. Here, we analyze an RB homolog, tobacco RETINOBLASTOMA-RELATED1 (NtRBR1), that encodes a protein possessing 13 potential phosphorylation sites for cyclin-dependent kinases (CDKs). We found that 11 synthetic peptides containing sequences from NtRBR1 were phosphorylated differentially by imunoprecipitates of cyclin/CDK complexes extracted from tobacco BY-2 cells. We raised antibodies that specifically recognize these sites of phosphorylation in NtRBR1. Distinct sites were phosphorylated during the cell cycle, suggesting that NtRBR1 is phosphorylated by different types of cyclin/CDK complexes.
Journal
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- Plant Biotechnology
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Plant Biotechnology 23 (5), 467-473, 2006
Japanese Society for Plant Biotechnology
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Keywords
Details 詳細情報について
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- CRID
- 1390282679304044800
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- NII Article ID
- 10021909454
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- NII Book ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 8571150
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed