Transgenic strawberry expressing the taste-modifying protein miraculin

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  • Sugaya Toshiyuki
    Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Yano Megumu
    Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Sun Hyeon-Jin
    Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Hirai Tadayoshi
    Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Ezura Hiroshi
    Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba

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Description

The gene encoding the taste-modifying protein miraculin was introduced under the control of the 35S or El2 promoter into strawberry (Fragaria x ananassa) by Agrobacterium-mediated transformation to produce transgenic plants. Although miraculin was detected in the leaves and fruits of the transgenic plants, the level of accumulation among the transgenic lines, which ranged from 0.5 to 2.0 μg g−1 fresh fruit, was not significantly different and was lower than that in miracle fruits (145 μg g−1 fresh fruit). High levels of miraculin accumulation were detected in the mature fruits. The transgenic lines were subsequently propagated via the runners for three vegetative generations, and miraculin was detected at equal levels in the leaves and fruits of the plants from each generation. In conclusion, although the level of accumulation was not high, miraculin was stably expressed and accumulated in the vegetative progeny of the transgenic strawberry plants.

Journal

  • Plant Biotechnology

    Plant Biotechnology 25 (4), 329-333, 2008

    Japanese Society for Plant Biotechnology

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