Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.) and field performance of somatic embryo-derived trees

DOI NDLデジタルコレクション Web Site Web Site 被引用文献1件 参考文献44件 オープンアクセス
  • Hosoi Yoshihisa
    Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute
  • Kuramoto Noritsugu
    Forestry and Forest Products Research Institute, Forest Tree Breeding Center Kyushu Regional Breeding Office
  • Maruyama Tsuyoshi E.
    Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute

書誌事項

タイトル別名
  • Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.) and field performance of somatic embryo-derived trees
公開日
2015
DOI
  • 10.5511/plantbiotechnology.15.0408a
公開者
日本植物バイオテクノロジー学会

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説明

In this study, 100 random amplified polymorphic DNA (RAPD) primers were screened to assess clonal fidelity in somatic embryos of Sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.). Of the 100 primers tested, eight were selected based on their amplification products, which showed clear DNA fragmentation, generating 8.75 bands per primer in average. The amplification products showed no genetic variation among the tested somatic embryos. In addition, the performance of somatic embryo-derived trees was monitored in the field, and their mean height growth was measured until the age of 10 years. Although no phenotypic differences were observed, the somatic embryo-derived trees showed lower growth compared with seedlings.

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