- 【Updated on May 12, 2025】 Integration of CiNii Dissertations and CiNii Books into CiNii Research
- Trial version of CiNii Research Knowledge Graph Search feature is available on CiNii Labs
- Suspension and deletion of data provided by Nikkei BP
- Regarding the recording of “Research Data” and “Evidence Data”
The <i>axhs1</i>/<i>dwf4</i> auxin-hypersensitive mutant of <i>Arabidopsis thaliana</i> defines a link for integration of auxin and brassinosteroid mediated root elongation
-
- Nagase Taisuke
- Department of Bioscience and Biotechnology, Faculty of Bioenvironmental Science, Kyoto Gakuen University
-
- Takase Hisabumi
- Department of Bioscience and Biotechnology, Faculty of Bioenvironmental Science, Kyoto Gakuen University
-
- Sekiya Jiro
- Department of Bioscience and Biotechnology, Faculty of Bioenvironmental Science, Kyoto Gakuen University
-
- Prieto Rafael
- Department of Bioscience and Biotechnology, Faculty of Bioenvironmental Science, Kyoto Gakuen University
Bibliographic Information
- Other Title
-
- The axhs1/dwf4 auxin-hypersensitive mutant of Arabidopsis thaliana defines a link for integration of auxin and brassinosteroid mediated root elongation
Search this article
Description
To understand the molecular mechanism of auxin-mediated root elongation, we carried out screening to isolate auxin-hypersensitive (axhs) mutants. The T-DNA insertional mutant axhs1 was selected on the basis of root phenotypes associated with auxin sensitivity. Results from brassinosteroid (BR) feeding experiments, Tail-PCR and genetic analyses indicate that AXHS1 encodes DWF4, which catalyzes the rate limiting step of BR biosynthesis. The axhs1 mutant shows increased sensitivity to indole-3-acetic acid (IAA), the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D), auxin transport inhibitors such as 2,3,5-triiodobenzoic acid (TIBA) and N-1-naphthylphthalamic acid (NPA), and the antiauxin p-chlorophenoxyisobutyric acid (PCIB) for the root elongation response. Analysis of the expression of the DR5:GUS and HS:AXR3NT-GUS reporter genes in wild type and axhs1 genotypes, and characterization of double mutants between axhs1 and mutants affected in auxin biosynthesis (wei2-1), auxin transport (aux1-7, eir1-1) and auxin signal transduction (tir1-1, axr1-3, axr2-1) indicate that auxin hypersensitivity in axhs1 is mediated by the auxin-signaling pathway and an AUX1, EIR1/PIN2 dependent auxin uptake.
Journal
-
- Plant Biotechnology
-
Plant Biotechnology 32 (2), 125-137, 2015
Japanese Society for Plant Biotechnology
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282679304227712
-
- NII Article ID
- 130005085997
-
- NII Book ID
- AA11250821
-
- ISSN
- 13476114
- 13424580
-
- NDL BIB ID
- 026560164
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL Search
- Crossref
- CiNii Articles
- OpenAIRE
-
- Abstract License Flag
- Disallowed