Identification of an S-RNase binding protein1 (SBP1) homolog of apple (<i>Malus</i>×<i>domestica</i>)
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- Minamikawa Mai F.
- Graduate School of Horticulture, Chiba University
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- Fujii Daisuke
- Graduate School of Horticulture, Chiba University
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- Kakui Hiroyuki
- Graduate School of Horticulture, Chiba University
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- Kotoda Nobuhiro
- Apple Breeding and Physiology Research Team, National Institute of Fruit Tree Science
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- Sassa Hidenori
- Graduate School of Horticulture, Chiba University
Bibliographic Information
- Other Title
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- Identification of an S-RNase binding protein1 (SBP1) homolog of apple (Malus×domestica)
- Identification of an S-RNase binding protein1 (SBP1) homolog of apple (Malus^|^times;domestica)
- Identification of an S-RNase binding protein1 (SBP1) homolog of apple (<italic>Malus</italic> × <italic>domestica</italic>)
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Abstract
Many flowering plants exhibit self-incompatibility (SI) to prevent inbreeding and promote outcrossing. This self/non-self discrimination mechanism is controlled by the S locus, which contains separate genes for pistil and pollen specificities. In the gametophytic SI (GSI) of Rosaceae, Solanaceae and Plantaginaceae, the pistil S determinant, S-RNase, encodes extracellular ribonuclease which is thought to act as a cytotoxin to the self pollen tube, while the pollen S determinant is the F-box gene called SLF/SFB/SFBB. In Petunia (Solanaceae), SLF is reported to be a component of the noncanonical E3 ubiquitin ligase complex with S-RNase binding protein1 (SBP1) and Cullin1 (CUL1), and interact with non-self S-RNases to ubiquitinate them for degradation. Here, we isolated an apple (Malus×domestica) homolog of SBP1 (MdSBP1) from pollen RNA by RT-PCR. MdSBP1 included a RING-HC domain required for E3 ubiquitin ligase activity, and showed 64.0–68.2% amino acid identities with solanaceous SBP1 proteins. Expression analysis showed that MdSBP1 was expressed in all the organs analyzed. We detected an interaction between recombinant MdSBP1 protein and S-RNase of apple using a pull-down assay.
Journal
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- Plant Biotechnology
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Plant Biotechnology 30 (2), 119-123, 2013
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390282679304259584
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- NII Article ID
- 10031184115
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- NII Book ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BC3sXhtlCqsL3O
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 024676539
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed