High-level gene expression in differentiating xylem of tobacco driven by a 2.0kb Poplar COMT2 promoter and a 4×35S enhancer

DOI Web Site 参考文献82件
  • Liu Enying
    Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University
  • Peng Shaobing
    College of Forestry, Northwest A&M University
  • Li Quanzi
    Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University College of Forestry, Shandong Agricultural University
  • Sun Ying-Hsuan
    Department of Forestry, National Chung Hsing University
  • Chiang Vincent L.
    Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University
  • Sederoff Ronald R.
    Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University

書誌事項

タイトル別名
  • High-level gene expression in differentiating xylem of tobacco driven by a 2.0 kb <i>Poplar COMT2</i> promoter and a 4×35S enhancer
  • High-level gene expression in differentiating xylem of tobacco driven by a 2.0^|^#8201;kb Poplar COMT2 promoter and a 4^|^times;35S enhancer

この論文をさがす

抄録

Promoter constructs with high levels of xylem specific expression are needed to obtain efficient expression of candidate genes, microRNAs (miRNAs) and artificial microRNAs (amiRNAs) for the genetic modification of wood properties. The gene for caffeic acid O-methytransferase (PtrCOMT2) has the second most abundant transcript level of all the genes in monolignol biosynthesis in Populus trichocarpa and a high level of specificity in differentiating xylem. To characterize the PtrCOMT2 promoter, we cloned a short (2.0 kb) and a long (3.3 kb) promoter segment and compared their expression using GUS as a reporter gene in the differentiating xylem of Nicotiana tabacum. Both the 2.0 kb and the 3.3 kb promoter segments showed high specificity for differentiating xylem in this heterologous system. GUS activity increased as much as 5 times when the 4×35S enhancer was inserted in front of the 2.0 kb promoter, but GUS activity was only increased 2 times when the enhancer was inserted behind the promoter. The enhancer inserted upstream reduced the expression of the 3.3 kb promoter. While expression of some of the enhancer-plus-promoter constructs increased expression, there was a loss of specificity.

収録刊行物

参考文献 (82)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ