G-to-A mutation at a 5' splice site of fad3c caused impaired splicing in a low linolenic mutant of canola (Brassica napus L.)
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- Hu Xueyi
- Dow AgroSciences, LLC
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- Sullivan-Gilbert Mandy
- Dow AgroSciences, LLC
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- Gupta Manju
- Dow AgroSciences, LLC
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- Thompson Steven A.
- Dow AgroSciences, LLC
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Two genes (fad3a and fad3c) encode for endoplasmic delta-15 linoleate desaturase, which is responsible for the desaturation of linoleic acid (C18:2) into linolenic acid (C18:3) in canola (Brassica napus). The canola mutant line DMS100 carries a G-to-A base substitution at the 5′ splice site, located at +1 G, of fad3c intron 6 and contains reduced C18:3 content in oil seeds. Reverse transcription (RT) PCR analysis was used to amplify part of the fad3c transcript spanning intron 5, exon 6 and intron 6 using the primers specific to the exon 5 and 7 to investigate whether the mutation caused any abnormal splicing. The RT-PCR amplified a fragment of larger size in the fad3c mutant than in wild-type. Sequencing of the RT-PCR fragments revealed that the mutation activated an impaired splicing which retained the entire intron 6 in the mature fad3c transcript of the mutant. The impaired splicing could result in early termination of translation and synthesis of a shorter fad3c polypeptide because the intron 6 contained stop codons in all three possible reading frames. The incomplete translation could produce an inactive enzyme which blocked the desaturation of linoleic acid (C18:2) to linolenic acid (C18:3), resulting in the decrease of C18:3 accumulation in canola seeds. This is consistent with the observation that the fad3c mutant has significantly lower C18:3 content (<3%) than the wild type (~7%).
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 24 (4), 397-400, 2007
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679304460928
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- NII論文ID
- 10021911864
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD2sXhtF2rtrrM
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 8904930
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 使用不可