Isolation and expression analysis of candidate genes related to Ralstonia solanacearum-tobacco interaction

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  • Kiba Akinori
    Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
  • Maimbo Milimo
    Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
  • Kanda Ayami
    Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
  • Tomiyama Hiromi
    Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
  • Ohnishi Kouhei
    Research institute of Molecular Genetic, Kochi University
  • Hikichi Yusufumi
    Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University

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  • Isolation and expression analysis of candidate genes related to <italic>Ralstonia</italic> <italic>solanacearum</italic> – tobacco interaction

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Abstract

Ralstonia solanacearum is a causal agent of bacterial wilt, and the bacterium normally infects through wounds in roots. Since it is not possible to equally and simultaneously inoculate the host plants with bacteria via root inoculation, it has proven difficult to elucidate the molecular events in plants infected with R. solanacearum. To improve the efficiency of inoculation, we inoculated tobacco plants with R. solanacearum by leaf-infiltration. Differential display was carried out to isolate fragments of genes that are regulated in tobacco by virulent strains of R. solanacearum OE1-1 and by the avirulent mutant of R. solanacearum, 31b, which is mutated in the type III secreted PopA protein by transposon insertion. Nineteen R. solanacearum-responsive genes (RsRGs) were successfully isolated using equalized cDNA libraries constructed with water-, R. solanacearum OE1-1- and 31b-infiltrated tobacco leaves. From Northern blot analysis, RsRGs were divided into 3 groups; 1) responsive to both virulent and avirulent bacteria (8 RsRGs), 2) responsive to avirulent bacteria (3 RsRGs), and 3) responsive to virulent bacteria (3 RsRGs). These results suggest that the cDNA pool and method presented in this study provide a valuable resource for functional genomic analysis of R. solanacearum-plant interactions.

Journal

  • Plant Biotechnology

    Plant Biotechnology 24 (4), 409-416, 2007

    Japanese Society for Plant Biotechnology

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