Expression of Arabidopsis thaliana cytochrome P450 monooxygenase, CYP71A12, in yeast catalyzes the metabolism of herbicide pyrazoxyfen

DOI Web Site 被引用文献2件 参考文献18件
  • Hayashi Etsuko
    Clinical Research Department 3, Development & Medical Affairs Division, GlaxoSmithKline K.K.
  • Fuzimoto Kenji
    Functional Analysis of Environmental Genes Research Center for Environmental Genomics, Kobe University
  • Imaishi Hiromasa
    Functional Analysis of Environmental Genes Research Center for Environmental Genomics, Kobe University

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In higher plants, cytochrome P450 (P450, CYP) monooxygenases play important roles in the biosynthesis and metabolism of endogenous compounds and/or in the oxidative reactions of xenobiotics, such as herbicides, insecticides, and fungicides. In this study, the novel P450 cDNAs encoding CYP71A12 was isolated from wild-type Arabidopsis thaliana ecotype Ws using an RT-PCR strategy. Northern blot analysis revealed that cyp71A12 is expressed specifically in roots but not in flowers, leaves, or stems. To determine the function of CYP71A12, the coding sequences of the corresponding cDNAs were expressed in Saccharomyces cerevisiae AH22 cells. Microsomal fractions expressing CYP71A12 metabolized the herbicide pyrazoxyfen. Liquid chromatography mass spectrometry (LC/MS) analysis indicated that the metabolite of pyrazoxyfen might be N-demethylated at the pyrazole ring and 5-hydroxylated at the dichlorobenzene ring. This is the first report that a pyrazole herbicide is metabolized by a plant P450 species.

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