Long-term Storage and Cryopreservation of Desiccated Microspore-derived Embryos in Brassica spp.
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- WAKUI Kenji
- Faculty of Agriculture, Iwate University
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- SATO Chiseko
- Faculty of Agriculture, Iwate University
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- TAKAHATA Yoshihito
- Faculty of Agriculture, Iwate University
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- KAIZUMA Norihiko
- Faculty of Agriculture, Iwate University
書誌事項
- タイトル別名
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- Long term Storage and Cryopreservation
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抄録
Microspore-derived embryos of Brassica napus were cryopreserved in liquid nitrogen (LN) after desiccation treatment. Desiccated embryos which were directly immersed in LN converted to plantlets without any decrease in their viability. In contrast, non-desiccated embryos lost their viability after cryopreservation. The longevity and viability of the desiccated embryos of B. campestris were examined under several storage conditions. Survival rates of the embryos were affected by temperature of storage. Lower temperature conditions prolonged their viability. Storage up to 3 years at -80°C did not affect the plant conversion ability of desiccated embryos. These results suggest that naked desiccated embryos of Brassica spp. could be stored for a long period at lower-temperature.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 15 (3), 123-126, 1998
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679304508800
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- NII論文ID
- 10011046944
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 4556166
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可