Molecular Cloning of cDNA for a Novel Pea Dof Protein, PsDof1, and Its DNA-Binding Activity to the Promoter of PsDof1 Gene.
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- SEKI Hikaru
- Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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- NAKAMURA Naoki
- Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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- MARUTANI Mizuri
- Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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- OKABE Takeharu
- Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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- SANEMATSU Shiroh
- Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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- INAGAKI Yoshishige
- Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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- TOYODA Kazuhiro
- Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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- SHIRAISHI Tomonori
- Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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- YAMADA Tetsuji
- Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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- ICHINOSE Yuki
- Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
Bibliographic Information
- Other Title
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- Molecular cloning of cDNA for a novel pea Dof protein, PsDof1, and its DNA binding activity to the promoter of PsDofl gene
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Abstract
A cDNA clone, designated PsDof1, encoding a novel member of the Dof DNA-binding protein family was isolated from pea. Random-binding-site selection and gel retardation experiments showed that the GST-PsDof1 recombinant protein bound to specific DNA sequences with an AAAG core. A metal-chelating agent, 1, 10-phenanthroline, abolished binding of GST-PsDof1 to DNA, but the addition of Zn2+ restored it, indicating that zinc ions are required for its DNA-binding activity. Interestingly, there are possible sites for binding PsDof1 in its promoter sequence. Indeed, GST-PsDof1 binds to the AAAG-containing promoter sequence of the PsDof1 gene with higher affinity. These results suggest that PsDof1 is a novel transcription factor that is involved in its own transcriptional regulation.
Journal
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- Plant Biotechnology
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Plant Biotechnology 19 (4), 251-260, 2002
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390282679304522880
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- NII Article ID
- 10010149329
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- NII Book ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD38XpslaqsrY%3D
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 6373710
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed