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Identification of a xylem sap germin-like protein and its expression under short-day and non-freezing low-temperature conditions in poplar root

  • Aohara Tsutomu
    Faculty of Life and Environmental Sciences, University of Tsukuba
  • Mizuno Hiroaki
    Faculty of Life and Environmental Sciences, University of Tsukuba
  • Kiyomichi Daiki
    Faculty of Life and Environmental Sciences, University of Tsukuba
  • Abe Yuta
    Faculty of Life and Environmental Sciences, University of Tsukuba
  • Matsuki Kaoru
    Faculty of Life and Environmental Sciences, University of Tsukuba
  • Sagawa Keiko
    Faculty of Life and Environmental Sciences, University of Tsukuba
  • Mori Hitoshi
    Graduate School of Bioagricultural Sciences, Nagoya University
  • Iwai Hiroaki
    Faculty of Life and Environmental Sciences, University of Tsukuba
  • Furukawa Jun
    Faculty of Life and Environmental Sciences, University of Tsukuba
  • Satoh Shinobu
    Faculty of Life and Environmental Sciences, University of Tsukuba

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Abstract

In the shoots of photoperiod-sensitive deciduous trees, including poplar, short-day and non-freezing low-temperature conditions induce bud dormancy and its break, respectively, and these conditions also induce shoot cold acclimation. In a previous study, levels of organic and inorganic components, including proteins, increased in the xylem sap of Populus nigra in winter, suggesting seasonal changes in root functions. Here, analysis of a major xylem sap protein (XSP24) of P. nigra in winter by mass spectrometry together with the whole genome sequence of P. trichocarpa and transcript abundance in roots under short-day conditions identified PtXSP24 to be a germin-like protein of the cupin superfamily, which was reported to be associated with various stresses and to have oxalate oxidase and/or superoxide dismutase activities in the cell wall. Expression of XSP24, which corresponds to PtXSP24 in P. maximowiczii, a potentially useful Japanese native poplar in the same phylogenetic clade as P. trichocarpa, was enhanced under short-day and non-freezing low-temperature conditions, as well as by application of abscisic acid. These results suggest that XSP24 is involved in tolerance to environmental stresses in autumn and early winter.

Journal

  • Plant Biotechnology

    Plant Biotechnology 33 (2), 123-127, 2016

    Japanese Society for Plant Biotechnology

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