A Cybrid Plant Produced by Electrofusion between Citrus unshiu(satsuma mandarin) and C. sinensis(sweet orange).

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  • 電気融合法によるウンシュウミカン (<i>Citrus unshiu</i>) とスイートオレンジ (<i>C. sinensis</i>) の細胞質雑種の作出
  • 電気融合法によるウンシュウミカン(Citrus unshiu)とスイートオレンジ(C.sinensis)の細胞質雑種の作出〔英文〕
  • デンキ ユウゴウホウ ニヨル ウンシュウ ミカン Citrus unshiu

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Electrofusion was conducted to combine Citrus unshiu cv. ‘Juman’ unshiu protoplasts isolated from embryogenic callus with C. sinensis cv. ‘F. N. Washington’ navel orange mesophyll protoplasts. One plant was regenerated from the fusion products. The plant has 18 chromosomes (2n=18 in each parent), and it showed the same nuclear rDNA fragment pattern as that of C. sinensis. Whereas, chloroplast and mitochondrial DNA analyses showed that the plant contained C. unshiu chloroplast and mitochondrial genomes. From these results, the regenerated plant was confirmed as a cybrid having C. sinensis nuclear genome and C. unshiu cytoplasmic genome.

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