Efficient Acquisition of Apple Rootstock Seedlings by In Vitro Ovule Culture.

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  • OOMIYA Tomo
    Faculty of Agriculture, Iwate University Current address: Takikawa Animal Husbandrg Experiment Station of Hokkaido
  • ISHIHARA Aiya
    Faculty of Agriculture, Iwate University

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  • リンゴ台木マルバカイドウにおけるはい珠培養を利用した効率的な実生育成
  • リンゴ ダイギ マルバカイドウ ニ オケル ハイシュ バイヨウ オ リヨウシタ

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Abstract

Seedlings of the apple rootstock Marubakaidou ‘Morioka-seishi’ (Malus prunifolia Bork. var. ringo Asami) were efficiently obtained by in vitro ovule culture. Ovules with placenta and core tissue segment were taken from open-pollinated fruits of various mature stages (10, 17 or 24 days after full-bloom) and cultured on modified Nitsch's (1969) media containing various concentrations of sucrose (12 to 25%) in the dark. After 110 days, developing embryos were excised from the cultured ovule and transferred to halfstrength LS medium containing 1mg/l BA and 2mg/l GA4 under continuous illumination for germination. Frequencies of both embryo development in the cultured ovule and seedling formation increased as more mature fruits were used. Seedlings were obtained most efficiently (50%) when ovules containing early globular embryo (24 days after full-bloom) were cultured on the medium with 15% sucrose. However, few seedlings were obtained from immature ovules containing proembryo when cultured on the media with 21 or 25% sucrose.

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