Isolation and Characterization of Sea Aster Salt-Stress Responsive Cysteine Protease Gene Obtained by a Hetero-probed Macroarray
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- TAKEDA Migiwa
- Graduate School of Science and Technology, Kobe University
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- KUWATA Chikara
- Kazusa DNA Research Institute
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- UNO Yuichi
- Faculty of Agriculture, Kobe University
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- KANECHI Michio
- Graduate School of Science and Technology, Kobe University
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- SHIBATA Daisuke
- Kazusa DNA Research Institute
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- INAGAKI Noboru
- Graduate School of Science and Technology, Kobe University
Bibliographic Information
- Other Title
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- Isolation and characterization of sea aster salt-stress responsive cystein protease gene obtained by a hetero-probed macroarray
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Abstract
To isolate and analyze salt-stress inducible genes in a halophyte, sea aster (Aster tripolium L.), we screened 5760 Arabidopsis cDNA clones by macroarray procedure using 33P-labeled cDNA targets synthesized from mRNAs isolated from NaCl treated and untreated sea aster seedlings. Seventeen Arabidopsis cDNAs were hetero-hybridized to NaCl inducible sea aster genes. These cDNAs were used as probes to isolate cDNA homologs from a sea aster cDNA library. One of the obtained cDNAs shared 71% amino acids identity with Arabidopsis cysteine protease (AtCysP) and named SaCysP (sea aster CysP). Northern blot analysis revealed that mRNAs corresponding to both SaCysP and AtCysP were induced by salt and osmotic stress in leaves. On the other hand, SASR21 mRNA encoding another CysP in sea aster was irresponsive to these stress in leaves but respond in roots. SaCysP and SASR21 genes may have a tissue-specific function in stress response by modulating their expression levels.
Journal
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- Plant Biotechnology
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Plant Biotechnology 21 (2), 127-133, 2004
Japanese Society for Plant Biotechnology
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Keywords
Details 詳細情報について
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- CRID
- 1390282679305301248
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- NII Article ID
- 130000064310
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- NII Book ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD2cXlvVGrtbc%3D
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 6960246
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed