Molecular and Cytogenetic Characterization of Triploid Somatic Hybrids between 'Shogun' Mandarin and Grapefruit.

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  • KUNITAKE Hisato
    Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University
  • NAGASAWA Kohji
    Laboratory of Pomology, School of Agriculture, Kyushu Tokai University
  • TAKAMI Kayo
    Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University
  • KOMATSU Haruki
    Laboratory of Pomology, School of Agriculture, Kyushu Tokai University

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Description

Somatic hybrids were produced between embryogenic callus protoplasts of ‘Shogun’ mandarin (Citrus reticulata Blanco, 2n = 2x = 18) and leaf protoplasts of grapefruit (C. paradisi Macf., 2n = 2x =18) by electrofusion. Hybridity of the two regenerated plants was confirmed by leaf characteristics and nuclear genome analysis using RAPD and PCR-RFLP. Flow cytometry and root-tip cell count of the two hybrids revealed a chromosome number of 27. These triploid somatic hybrids on transfer to field showed normal morphology and vigorous growth. Field performances as well as disease resistance are under investigation.

Journal

  • Plant Biotechnology

    Plant Biotechnology 19 (5), 345-352, 2002

    Japanese Society for Plant Biotechnology

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