An efficient transient gene expression system using aleurones of diploid wheat seeds
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- Utsugi Shigeko
- Research Institute for Bioresources, Okayama University
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- Maekawa Masahiko
- Research Institute for Bioresources, Okayama University
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- Noda Kazuhiko
- Research Institute for Bioresources, Okayama University
書誌事項
- タイトル別名
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- efficient transient gene expression system using aleurones of diploid wheat seeds
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To establish a transient gene expression system for investigating the molecular function of genes in mature wheat seeds, we bombarded embryos or aleurones of three hexaploid wheat (Triticum aestivum L.) varieties Chinese Spring, RL4137 and OS21-5, two diploid wheat (T. monococcum L.) varieties KT3-1 and KT3-5 and a barley (Hordeum vulgare, L.) variety Himalaya with fusion constructs containing the GUS reporter gene driven by the Actin1 promoter or α-amylase promoter using a particle gun, and compared the efficiency of gene expression for the transient assay. The efficiency of transient expression was high in aleurone tissues of OS21-5, KT3-1, KT3-5 and Himalaya. In the aleurone tissues of KT3-5, the α-amylase promoter was especially markedly activated by gibberellic acid (GA) and by a GA-inducible MYB transcription activator of T. monococcum, TmGAMYB. This assay system using the aleurone tissues of KT3-5 may be especially useful for investigating which genes are expressed and how they are regulated in germinating wheat seeds.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 23 (4), 413-417, 2006
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679305806976
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- NII論文ID
- 10021909246
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD28XhtFSjsr%2FM
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- ISSN
- 13476114
- 13424580
- http://id.crossref.org/issn/13424580
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- NDL書誌ID
- 8041323
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 使用不可