Cloning and molecular analysis of radish (Raphanus sativus L.) cDNAs encoding heterodimeric .GAMMA.-glutamyltransferases
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- Nakano Yoshihiro
- Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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- Prieto Rafael
- Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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- Hara Masakazu
- Faculty of Agriculture, Shizuoka University
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- Sekiya Jiro
- Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Bibliographic Information
- Other Title
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- Cloning and molecular analysis of radish (Raphanus sativus L.) cDNAs encoding heterodimeric γ-glutamyltransferases
- Cloning and molecular analysis of radish Raphanus sativus L cDNA s encoding heterodimeric ガンマ glutamyltransferases
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Abstract
γ-Glutamyltransferase (GGT) catalyzes the hydrolysis and transpeptidation of the γ-glutamyl moiety of γ-glutamyl peptides. Based on the N-terminal amino acid sequences of purified radish heterodimeric GGTs, we cloned and characterized three radish full-length cDNAs (RsGGT1, RsGGT2 and RsGGT3) encoding putative heterodimeric GGT isoforms. RsGGT proteins contained conserved amino acid residues that are required for the catalytic activity and the post-translational processing of GGT proteins in E. coli and mammals. Expression analysis indicated that RsGGT showed different organ expression patterns. The overexpression of RsGGT1 and RsGGT2 cDNAs, but not that of RsGGT3 cDNA, resulted in an increase of NaCl-extractable bound GGT activity in transgenic tobacco plants. These results suggest that RsGGT1 and RsGGT2 cDNAs encode heterodimeric bound GGT isoforms.
Journal
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- Plant Biotechnology
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Plant Biotechnology 23 (4), 419-424, 2006
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390282679305808256
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- NII Article ID
- 10021909262
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- NII Book ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD28XhtFSjsr%2FN
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 8041332
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed