Identification of Novel Microsporogenesis - Associated Genes Encoding Proteins with a Nuclear Localization Signal.

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  • BAYARMAA Gun-Aajav
    Graduate School of Biological Sciences, Nara Institute of Science and Technology
  • MOROHASHI Kengo
    Division of Plant Biotechnology, Graduate School of Environment and Information Sciences, Yokohama National University
  • TAKASE Hisabumi
    Graduate School of Biological Sciences, Nara Institute of Science and Technology
  • HIRATSUKA Kazuyuki
    Division of Plant Biotechnology, Graduate School of Environment and Information Sciences, Yokohama National University

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Abstract

Microsporogenesis is a highly organized indispensable event for the sexual reproduction of higher plants. The process includes a series of organ development, cell differentiation and meiosis. We examined the functional genes involved in microsporogenesis by random sequencing using a cDNA library from lily zygotene stage microsporocytes and isolated two cDNAs encoding novel gene products. Deduced amino acid sequences of both gene products, designated M355 and M404 contained a cluster of basic amino acid residues that may constitute a nuclear loclization signal. RT-PCR analysis indicated that the temporal and spatial expression of M355 and M404 is associated with early stages of microsporogenesis or meiosis. Transient expression of GFP fusion proteins in onion epidermal cells revealed nuclear localization activity of both proteins. These results suggest that M355 and M404 are involved in a nuclear event during the progression of microsporogenesis in lily.

Journal

  • Plant Biotechnology

    Plant Biotechnology 20 (2), 137-143, 2003

    Japanese Society for Plant Biotechnology

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