<i>N</i>-Glycan structures of glycoproteins in suspension-cultured <i>Arabidopsis thaliana</i> T87 cells

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  • N-Glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana T87 cells

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Abstract

Suspension-cultured plant cells are feasible bioreactors for the production of pharmaceutical proteins. Post-translational modification, such as glycosylation, is important for in vivo biological and physiological roles of proteins. However, little is known about the effect of suspension-culture conditions on protein N-glycosylation. In this study, we studied the N-glycan structures of soluble and endogenous glycoproteins at different growth stages of suspension-cultured Arabidopsis thaliana T87 cells. At the late growth stage, the amount of N-glycans with terminal N-acetylglucosamine (GlcNAc) decreased, whereas that of N-glycans with fucose and xylose increased. This indicates that N-glycans have more plant-specific sugar residues as the cultivation reaches the late or end stage of plant growth.

Journal

  • Plant Biotechnology

    Plant Biotechnology 29 (5), 489-493, 2012

    Japanese Society for Plant Biotechnology

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