Involvement of a tobacco leucine-rich repeat-extensin in cell morphogenesis

DOI 1 Citations 32 References
  • Chida Hiroshi
    Graduate School of Life and Environmental Science, University of Tsukuba
  • Yazawa Katsumi
    Graduate School of Life and Environmental Science, University of Tsukuba
  • Hasezawa Seiichiro
    Graduate School of Frontier Sciences, University of Tokyo
  • Iwai Hiroaki
    Graduate School of Life and Environmental Science, University of Tsukuba
  • Satoh Shinobu
    Graduate School of Life and Environmental Science, University of Tsukuba

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Abstract

The unique mutant nolac-K4 (non-organogenic callus with loosely attached cells), which was generated by T-DNA transformation using leaf-disk cultures of haploid Nicotiana plumbaginifolia, has lost the ability to form adventitious shoots and also shows decreased intercellular attachment. The gene tagged with the T-DNA in this line, named NpLRX1 (LRR-EXTENSIN 1), is a novel tobacco gene that encodes a cell-wall protein containing chimeric leucine-rich repeat and extensin domains. The gene is highly similar to the Arabidopsis LRX genes, and phylogenetic analysis places it in the vegetative LRX clade. NpLRX1 is expressed ubiquitously in vegetative tissues, strongly in the leaf and root vascular bundles, and in emerging lateral roots and root tips. Tobacco leaf disks transformed with an NpLRX1-RNAi construct displayed aberrant adventitious buds and disorganized cell morphogenesis with large intercellular spaces. The shapes of NpLRX1-RNAi-transformed BY-2 cells were irregular, and the cells showed disorganized cortical microtubules. These results suggest that NpLRX1, the lack of which may be responsible for the nolac-K4 mutation, is a new tobacco LRX gene that has regulatory roles in cell morphogenesis that are essential for plant tissue development.

Journal

  • Plant Biotechnology

    Plant Biotechnology 24 (2), 171-177, 2007

    Japanese Society for Plant Biotechnology

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Details

  • CRID
    1390282679305912064
  • NII Article ID
    10021910924
  • NII Book ID
    AA11250821
  • DOI
    10.5511/plantbiotechnology.24.171
  • COI
    1:CAS:528:DC%2BD2sXkslSmsLs%3D
  • ISSN
    13476114
    13424580
  • NDL BIB ID
    8690298
  • Text Lang
    en
  • Data Source
    • JaLC
    • NDL
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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