Involvement of a tobacco leucine-rich repeat-extensin in cell morphogenesis
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- Chida Hiroshi
- Graduate School of Life and Environmental Science, University of Tsukuba
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- Yazawa Katsumi
- Graduate School of Life and Environmental Science, University of Tsukuba
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- Hasezawa Seiichiro
- Graduate School of Frontier Sciences, University of Tokyo
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- Iwai Hiroaki
- Graduate School of Life and Environmental Science, University of Tsukuba
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- Satoh Shinobu
- Graduate School of Life and Environmental Science, University of Tsukuba
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説明
The unique mutant nolac-K4 (non-organogenic callus with loosely attached cells), which was generated by T-DNA transformation using leaf-disk cultures of haploid Nicotiana plumbaginifolia, has lost the ability to form adventitious shoots and also shows decreased intercellular attachment. The gene tagged with the T-DNA in this line, named NpLRX1 (LRR-EXTENSIN 1), is a novel tobacco gene that encodes a cell-wall protein containing chimeric leucine-rich repeat and extensin domains. The gene is highly similar to the Arabidopsis LRX genes, and phylogenetic analysis places it in the vegetative LRX clade. NpLRX1 is expressed ubiquitously in vegetative tissues, strongly in the leaf and root vascular bundles, and in emerging lateral roots and root tips. Tobacco leaf disks transformed with an NpLRX1-RNAi construct displayed aberrant adventitious buds and disorganized cell morphogenesis with large intercellular spaces. The shapes of NpLRX1-RNAi-transformed BY-2 cells were irregular, and the cells showed disorganized cortical microtubules. These results suggest that NpLRX1, the lack of which may be responsible for the nolac-K4 mutation, is a new tobacco LRX gene that has regulatory roles in cell morphogenesis that are essential for plant tissue development.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 24 (2), 171-177, 2007
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679305912064
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- NII論文ID
- 10021910924
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD2sXkslSmsLs%3D
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 8690298
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDLサーチ
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- 使用不可