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Generation of gamma irradiation-induced mutant lines of the miniature tomato (Solanum lycopersicum L.) cultivar 'Micro-Tom'

  • Matsukura Chiaki
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Yamaguchi Isao
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Inamura Mami
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Ban Yusuke
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Kobayashi Yoshie
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Yin Yong-gen
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Saito Takeshi
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Kuwata Chikara
    Crop Breeding Institute, Chiba Prefectural Agriculture Research Center
  • Imanishi Shunsuke
    National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
  • Nishimura Shigeo
    Graduate School of Life and Environmental Sciences, University of Tsukuba

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Abstract

One direct way to identify a gene and its function is a forward genetic approach based on mutation analyses. To accumulate genetic resources for breeding and functional genomics in the tomato, we generated 6,347 lines of an M2 population with 300 Gy of gamma-ray irradiation in the inbred miniature dwarf variety ‘Micro-Tom.’ In total, 6,301 M2 lines were screened based on morphological alteration and brix-aberration, and 237 lines were selected as mutant candidates at the first screening. Subsequent screening of the self-fertilized M3 and M4 progeny yielded 24 lines of morphological mutants and 11 lines of aberrant brix mutants. Segregation data suggested that most of the mutant lines had single recessive mutations, with the exception of two lines. The chlorophyll mutation ratio in germinated M2 seedlings was 0.37% and the actual mutant frequency was 0.5%. The selected mutant lines exhibited a wide range of mutations, including whole plant properties with a severe phenotype, which allowed for more efficient screening of knockout mutants. Three characterized mutants, pale leaf, pink, and short root are also described.

Journal

  • Plant Biotechnology

    Plant Biotechnology 24 (1), 39-44, 2007

    Japanese Society for Plant Biotechnology

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Details

  • CRID
    1390282679305979136
  • NII Article ID
    130000064790
  • NII Book ID
    AA11250821
  • DOI
    10.5511/plantbiotechnology.24.39
  • ISSN
    13476114
    13424580
    http://id.crossref.org/issn/13424580
  • NDL BIB ID
    8690344
  • Text Lang
    en
  • Data Source
    • JaLC
    • NDL
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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