Isolation of Four RAD23 Genes from Arabidopsis thaliana and Detection of Alternative Splicing Variants
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- ISHIKAWA Yuichi
- Graduate School of Life Sciences, Tohoku University
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- ENDO Masaki
- Graduate School of Life Environmental Sciences, University of Tsukuba
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- ABE Kiyomi
- Department of Plant Biotechnology, National Institute of Agrobiological Sciences
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- OSAKABE Keishi
- Department of Plant Biotechnology, National Institute of Agrobiological Sciences
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- NAKAJIMA Nobuyoshi
- Biodiversity Conservation Research Project, National Institute for Environmental Studies
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- SAJI Hikaru
- Environmental Biology Division, National Institute for Environmental Studies
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- ITO Yuji
- Department of Plant Biotechnology, National Institute of Agrobiological Sciences
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- ICHIKAWA Hiroaki
- Department of Plant Biotechnology, National Institute of Agrobiological Sciences
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- KAMEYA Toshiaki
- Graduate School of Life Sciences, Tohoku University
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- TOKI Seiichi
- Department of Plant Biotechnology, National Institute of Agrobiological Sciences
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説明
DNA damage recognition during nucleotide excision repair (NER) involves the homologous heterodimers Rad4:Rad23 in budding yeast and XPC:hHR23B in human. We report here the characteristics of four Arabidopsis homologues of RAD23 gene, named AtRAD23-1 to-4. AtRAD23-1, -3 and -4 expressed two alternatively spliced transcripts, long ones (AtRAD23-1α, -3α and -4α) and short ones (AtRAD23-1β, -3β and -4β). The predicted amino acid sequences of these genes possessed four conserved domains of Rad23 family; the ubiquitin-like domain, ubiquitin-associated domain I, XPC-binding domain and ubiquitin-associated domainII. AtRad23-3 βand-4 βlacked the C-terminus ubiquitin-like domain and the C-terminus XPC-binding domain, respectively, suggesting that these alternatively spliced variants may modulate functional AtRad23 proteins. Phylogenetic analysis showed that plant RAD23 genes could be divided into two classes and that Arabidopsis RAD23 genes were recently duplicated. AtRAD23-1-4 transcripts were detected in various tissues, with the highest expression level in flower buds.
収録刊行物
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- Ｐｌａｎｔ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ
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Ｐｌａｎｔ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 21 (1), 65-71, 2004
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679306015744
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- NII論文ID
- 10012727205
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD2cXlslSls7g%3D
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 6884300
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- 本文言語コード
- en
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- データソース種別
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- NDLサーチ
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