Production of Triterpenes from the Callus Tissues of Actinidiaceous Plants.

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  • TAKAZAWA Hiroshi
    Department of Biological Science and Technology, Science University of Tokyo
  • YOSHIMURA Kouichi
    Department of Biological Science and Technology, Science University of Tokyo
  • IKUTA Akira
    Research Institutes for Science and Technology, Science University of Tokyo
  • KAWAGUCHI Kiichiro
    Medicinal Plant Garden, School of Pharmaceutical Science, KitasatoUniversity

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説明

Callus tissue from the stems of Actinidia arguta (Actinidiaceae) produced the following eight ursane-type triterpenes: α-amyrin; uvaol; ursolaldehyde; ursolic acid; corosolic acid; asiatic acid; 2α, 3β, 24-trihydroxyurs-12-en-28-oic acid (4-epiasiatic acid); and 2α, 3α, 24-trihydroxyurs-12-en-28-oic acid. The tissue also produced three oleanane-type triterpenes (β-amyrin, oleanolic acid, and maslinic acid), and two phytosterols mixtures (sitosterol and stigmasterol). Seven of the eight ursane-type triterpenes (the exception being asiatic acid), the three oleanane-type triterpenes, and the two phytosterols mixtures were also isolated from A. chinensis and A. polygama callus tissues. Variations in the production ratios of two of the triterpenes (ursolic acid and oleanolic acid) and the two phytosterols mixtures (sitosterol and stigmasterol) were compared among callus tissues from the three plant species.

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