Induction of Multiple shoots by Shoot Apex Culture in Magnolia obovata Thunb.

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  • NAKAMURA Kentaro
    Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology Present address: Sumitomo Forestry Co., Ltd. Tsukuba Research Institute
  • WAKITA Yohichi
    Department of Forest Science, Faculty of Agriculture, Utsunomiya University
  • YOKOTA Shinso
    Department of Forest Science, Faculty of Agriculture, Utsunomiya University
  • YOSHIZAWA Nobuo
    Department of Forest Science, Faculty of Agriculture, Utsunomiya University
  • IDEI Toshinaga
    Department of Forest Science, Faculty of Agriculture, Utsunomiya University

Bibliographic Information

Other Title
  • ホオノキの茎頂培養による多芽体誘導
  • ホオノキの茎頂培養による多芽体誘導〔英文〕
  • ホオノキ ノ ケイチョウ バイヨウ ニ ヨル タ ガタイ ユウドウ エイブン

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Description

Shoot apex of Magnolia obovata Thunb. was cultured on the modified Murashige and Skoog's (MS) medium supplemented with the combinations of IBA and BAP or NAA and BAP in various concentrations. Multiple shoots were induced successfully on the half strength MS reducing nitrate to one fourth (1/4N-1/2MS) medium containing 4μM IBA and 5μM BAP after 40 days of culture. The multiple shoots obtained were divided into small nodal explants and further subcultured on fresh medium. Many axillary buds were induced from the explant after 20 days of culture, and they succeedingly grew actively and developed multiple shoots. Culturing the axillary bud together with the stem tissue was effective for facilitating the induction of multiple shoots.

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