Horticultural characterization of a tetraploid transgenic plant of Tricyrtis sp. carrying the gibberellin 2-oxidase gene
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- Otani Masahiro
- Faculty of Agriculture, Niigata University
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- Ishibe Mitsuyo
- Faculty of Agriculture, Niigata University
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- Inthima Phithak
- Faculty of Agriculture, Niigata University Department of Biotechnology, Faculty of Science, Mahidol University
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- Supaibulwatana Kanyaratt
- Department of Biotechnology, Faculty of Science, Mahidol University
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- Mori Shiro
- College of Agriculture, Food and Environmental Sciences, Rakuno Gakuen University
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- Niki Tomoya
- National Institute of Floricultural Science
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- Nishijima Takaaki
- National Institute of Floricultural Science
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- Koshioka Masaji
- College of Bioresource Sciences, Nihon University
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- Nakano Masaru
- Faculty of Agriculture, Niigata University
書誌事項
- タイトル別名
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- Horticultural characterization of a tetraploid transgenic plant of <i>Tricyrtis</i> sp. carrying the gibberellin 2-oxidase gene
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The gibberellin 2-oxidase catalyzes the bioactive gibberellins or their immediate precursors to inactive forms. We have previously produced transgenic plants of the liliaceous plant Tricyrtis sp. containing the GA2ox gene from the linderniaceous plant Torenia fournieri. These transgenic plants showed dwarf phenotypes as expected but unfortunately had no flowers or only small, unopened flowers. Recently, one newly produced transgenic line (G2-55) formed fully opened flowers. G2-55 showed a moderately dwarf phenotype and the shoot length decreased to 63.4% of that of the control, non-transgenic plants. No significant differences in the number of flowers per shoot and in the flower size were observed between G2-55 and the control. Flow cytometry analysis and chromosome observation showed that G2-55 was tetraploid (2n=4x=52), whereas the other transgenic lines producing no or only small flowers were diploid (2n=2x=26) as the mother plant. Pollen fertility of G2-55 was 81.2% as determined by acetocarmine staining. The tetraploidy in G2-55 might be resulted from somaclonal variation of embryogenic calluses used as a target material for Agrobacterium-mediated transformation. The tetraploid transgenic plant G2-55 may be useable not only directly as a potted plant, but also as a material for further breeding of Tricyrtis spp.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 31 (4), 335-340, 2014
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679306105216
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- NII論文ID
- 130004700266
- 40020313518
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 026003768
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- CiNii Articles
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- 使用不可