Identification of a 23kDa protein (P23k) related to the sugar supply in germinating barley seeds

DOI Web Site 被引用文献2件 参考文献23件
  • Kidou Shin-ichiro
    Cryobiosystem Research Center, Faculty of Agriculture, Iwate University
  • Oikawa Ai
    Cryobiosystem Research Center, Faculty of Agriculture, Iwate University
  • Sasaki Naoko
    Cryobiosystem Research Center, Faculty of Agriculture, Iwate University
  • Yasuda Hiroshi
    Cryobiosystem Research Center, Faculty of Agriculture, Iwate University
  • Yamashita Tetsuro
    Department of Agro-Science, Faculty of Agriculture, Iwate University
  • Koiwa Hiroyuki
    Iwate Biotechnology Research Center
  • Kato Kiyoaki
    Department of Crop Science, Obihiro University of Agriculture and Veterinary Medicine
  • Ejiri Shin-ichiro
    Cryobiosystem Research Center, Faculty of Agriculture, Iwate University

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We identified a 23 kDa protein expressed abundantly in young barley seedlings; this protein was named P23k. P23k was shown to have evolved in barley and its allied species such as wheat, oat and rye, suggesting that P23k might be closely related to a specific biological trait of winter cereals. To determine the function of P23k, we examined the expression and localization of P23k and its mRNA in barley seedlings. P23k mRNA was markedly expressed in the scutellum of imbibed barley seeds, and was synchronously down-regulated with consumption of the starchy endosperm. In addition, ABA and sugar treatment of the scutellum showed that expression of P23k is highly dependent on the supply of glucose or sucrose, both of which are starch catabolites. Furthermore, immunocytochemical analysis showed that P23k is primarily localized around the membranes, where sugar translocation is active. These results indicate that P23k plays a role in sugar translocation in young barley seedlings.

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