A highly efficient AgarTrap method for genetic transformation of mature thalli of the liverwort <i>Marchantia polymorpha</i> L.
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- Tsuboyama-Tanaka Shoko
- Center for Bioscience Research and Education, Utsunomiya University
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- Nonaka Satoko
- Graduate School of Life and Environmental Sciences, University of Tsukuba
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- Kodama Yutaka
- Center for Bioscience Research and Education, Utsunomiya University
Bibliographic Information
- Other Title
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- A highly efficient AgarTrap method for genetic transformation of mature thalli of the liverwort Marchantia polymorpha L
- A highly efficient AgarTrap method for genetic transformation of mature thalli of the liverwort <italic>Marchantia polymorpha</italic> L
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Abstract
Marchantia polymorpha L. has been established as a model liverwort species for understanding plant evolution, and several genetic transformation technologies have been developed. Recently, we described a simplified Agrobacterium-mediated genetic transformation method termed AgarTrap (Agar-utilized Transformation with Pouring Solutions) that uses sporelings (S-AgarTrap) or gemmae/gemmalings (G-AgarTrap), both of which easily produce a sufficient number of independent transformants. In this study, we report the development of a novel AgarTrap protocol using mature pieces of liverwort thalli (T-AgarTrap). Optimal transformation efficiency was approximately 70%, and 100% transformation was achieved in a few experimental cases. The efficiency of T-AgarTrap was much greater than those of S- and G-AgarTrap methods. This highly efficient T-AgarTrap protocol seems to promote molecular research with M. polymorpha.
Journal
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- Plant Biotechnology
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Plant Biotechnology 32 (4), 333-336, 2015
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390282679306167424
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- NII Article ID
- 130005115900
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- NII Book ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 027012355
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed