Normal skin and identification of S phase cell in endothelical dermatosis.
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- MORIMOTO Yousuke
- 札幌医科大学皮膚科学教室
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- TANAKA Mayumi
- 札幌医科大学皮膚科学教室
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- SAGA Kenji
- 札幌医科大学皮膚科学教室
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- TAKAHASHI Makoto
- 札幌医科大学皮膚科学教室
Bibliographic Information
- Other Title
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- 正常皮膚および上皮性皮膚疾患におけるS期細胞の同定
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Abstract
We studied the cells in S-phase in various benign and malignant skin diseases such as psoriasis, seborrheic keratosis, actinic keratosis, Bowen's disease, extramammary Paget's disease, basal cell carcinoma, squamous cell carcinoma, malignant lymphoma, and malignant melanoma. Small pieces of excised skin tumor were incubated with 5-bromo-2'-deoxyuridine (BrdUrd), thymidine analogue, under three atmospheres of pressures in 95% O2 with 5% CO2. Anti-BrdUrd monoclonal antibody stained the nuclei which incorporated that substance. We analyzed labeling pattern as well as the counting of labeling indices. Malignant diseases such as squamous cell carcinoma, basal cell carcinoma, and Bowen's disease showed higher labeling indices than non-malignant specimens. As for the labeling pattern, malignant diseases lost the polarity of labeling and labeled cells were scattered randomly without forming germinative layers. On the other hand, non-malignant specimens showed the germinative layers. Therefore, high labeling indices together with the loss of polarity seemed characteristic for the malignancy.
Journal
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- Skin Cancer
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Skin Cancer 7 (3), 270-275, 1992
The Japanese Skin Cancer Society
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Details 詳細情報について
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- CRID
- 1390282679313918336
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- NII Article ID
- 130001079281
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- ISSN
- 18843549
- 09153535
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed