スナネズミ胚のバリン代謝
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- Obata Ryuichiro
- Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shinshu University
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- Tsujii Hirotada
- Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shinshu University
書誌事項
- タイトル別名
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- Valine Metabolism of Mongolian Gerbil (Meriones unguiculatus) Embryos
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抄録
Mongolian gerbil 1-cell embryos do not develop into blastocysts in vitro because of 8-cell block. Recently, we reported that 2-cell embryos of Mongolian gerbils developed into blastocysts in vitro in mM16 supplemented with valine. However, the role of valine in the in vitro development of Mongolian gerbil embryos is unclear. Thus, the objective of this study was to investigate of the role of valine in the in vitro development of Mongolian gerbil embryos. Mongolian gerbil embryos at the 1-cell, 2-cell, 8-cell, morula, and blastocyst stages were cultured in mM16 medium containing 14C-valine or 14C-glucose. A 2.5 mM NaOH solution was used as a trap for the evolved 14CO2. After incubation, all samples were analyzed using a liquid scintillation counter. The incorporation of 14C-valine significantly increased at the 8-cell stage. The oxidation of 14C-valine significantly increased at the 8-cell and blastocyst stages. The incorporation of 14C-glucose in mM16+valine was significantly higher than that in mM16 at the blastocyst stage. The oxidation of 14C-glucose in mM16 was significantly higher than that in mM16+valine at the 8-cell stage, although that in mM16+valine was significantly higher than that in mM16 at the blastocyst stage. The carbon skeleton of 14C-valine was metabolized into lipid. Our results suggest that valine was used and metabolized as an energy source in preimplantation Mongolian gerbil embryos.<br>
収録刊行物
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- 日本哺乳動物卵子学会誌
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日本哺乳動物卵子学会誌 27 (3), 144-149, 2010
日本卵子学会
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詳細情報 詳細情報について
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- CRID
- 1390282679314701696
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- NII論文ID
- 130004436780
- 10027595166
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- NII書誌ID
- AN10548943
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- ISSN
- 13475878
- 13417738
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- NDL書誌ID
- 10894255
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可