<i>In Vitro</i> Development of Porcine <i>In Vitro</i> Matured Oocytes Vitrified after Removal of Cytoplasmic Lipid Droplets
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- Momozawa Kenji
- School of Veterinary Medicine, Kitasato University
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- Iwasaki Haruka
- School of Veterinary Medicine, Kitasato University
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- Onoda Yuka
- School of Veterinary Medicine, Kitasato University
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- Hagiwara Rie
- School of Veterinary Medicine, Kitasato University
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- Mori Mariko
- School of Veterinary Medicine, Kitasato University
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- Fukuda Yoshinori
- School of Veterinary Medicine, Kitasato University
Bibliographic Information
- Other Title
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- ガラス化保存した局在化脂肪滴除去ブタ卵子の体外受精後の胚盤胞への発生成績
- In Vitro Development of Porcine In Vitro Matured Oocytes Vitrified after Removal of Cytoplasmic Lipid Droplets
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Abstract
The objective of the present study was to investigate the in vitro developmental competence of porcine in vitro matured (IVM) oocytes vitrified after removal of cytoplasmic lipid droplets (delipation). After vitrification and warming, the delipated porcine IVM oocytes were inseminated and subsequently cultured in vitro. The rate of development to the blastocyst stage of delipated, vitrified oocytes (5.9%) was significantly lower than that of control oocytes (untreated oocytes) (26.2%). We also examined the influence of delipation of porcine IVM oocytes on development to the blastocyst stage following in vitro fertilization (IVF). Delipated porcine IVM oocytes (not vitrified) were inseminated and subsequently cultured in vitro. The rates of development to the blastocyst stage were similar for delipated and undelipated oocytes (21.1% and 26.2%, respectively). The results of the present study showed that delipated, vitrified porcine IVM oocytes can develop to the blastocyst stage following IVF, though blastocyst formation rate was low, and that delipation of porcine IVM oocytes did not negatively affect their development to blastocyst stage.<br>
Journal
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- Journal of Mammalian Ova Research
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Journal of Mammalian Ova Research 30 (1), 36-40, 2013
JAPANESE SOCIETY OF OVA RESEARCH
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Keywords
Details 詳細情報について
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- CRID
- 1390282679314979712
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- NII Article ID
- 10031166255
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- NII Book ID
- AN10548943
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- ISSN
- 13475878
- 13417738
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- NDL BIB ID
- 024530836
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed