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Analysis of Physiological Process in Early Stage of Human Embryos after ICSI using Time-lapse Cinematography
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- Adachi Yumi
- Reproductive Centre, Mio Fertility Clinic
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- Takeshita Chie
- Reproductive Centre, Mio Fertility Clinic
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- Wakatsuki Yuka
- Reproductive Centre, Mio Fertility Clinic
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- Iwata Kyoko
- Reproductive Centre, Mio Fertility Clinic
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- Kato Yukari
- Reproductive Centre, Mio Fertility Clinic
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- Ueno Yukiho
- Reproductive Centre, Mio Fertility Clinic
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- Mio Yasuyuki
- Reproductive Centre, Mio Fertility Clinic
Bibliographic Information
- Other Title
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- 非侵襲的連続観察装置(Time-lapse cinematography)を用いたヒト初期胚発生過程の解析
- ヒシンシュウテキ レンゾク カンサツ ソウチ Time lapse cinematography オ モチイタ ヒト ショキ ハイ ハッセイ カテイ ノ カイセキ
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Description
In order to evaluate the physiological events in early stage of human embryos after intracytoplasmic sperm injection (ICSI), we have developed new embryo culture system on the stage of inverted microscope as a time-lapse cinematography. Time-lapse recording (Exposure time: 50 ms; Interval: 2 mins) commenced shortly after ICSI and continued up to 40 h. We examined a total of 65 oocytes obtained from 62 patients with ICSI. Fifty five (84.6%) of 65 oocytes showed 2PN/2PB fertilization and all of which cleaved. Out of 55 embryos cleaved, 42 (76.4%) developed into morphologically good quality embryos (GQE), while remaining 13 embryos were classified as poor quality embryos (PQE). There was no significant difference in the time course required for physiological events during early stage of embryos between GQE and PQE. Although no significant difference was observed in the pattern of nucleolar precursor body (NPB) distribution between GQE and PQE, the appearance of cytoplasmic halo during 2PN stage of embryos was significantly related to embryo quality (p<0.05). This study indicates that cytoplasmic halo is a new parameter in the evaluation of good quality embryo. Furthermore, time-lapse cinematography is an excellent tool in elucidating the physiological phenomenon in the early stage of human embryos.<br>
Journal
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- Journal of Mammalian Ova Research
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Journal of Mammalian Ova Research 22 (2), 64-70, 2005
JAPANESE SOCIETY OF OVA RESEARCH
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Keywords
Details 詳細情報について
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- CRID
- 1390282679315068544
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- NII Article ID
- 10015712265
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- NII Book ID
- AN10548943
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- ISSN
- 13475878
- 13417738
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- NDL BIB ID
- 7358403
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- Text Lang
- ja
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- Article Type
- journal article
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- Data Source
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- JaLC
- IRDB
- NDL Search
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed