Freeze-drying in Mouse Spermatozoa

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  • Kawase Yosuke
    Chugai Research Institute for Medical Science, Inc.
  • Suzuki Hiroshi
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine Department of Developmental and Medical Technology, Graduate School of Medicine, the University of Tokyo

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  • マウス精子の凍結乾燥
  • マウス セイシ ノ トウケツ カンソウ

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Abstract

Cryopreservation of mouse spermatozoa has been widely applied for maintenance of genetically modified mouse strains. Although cryopreservation of spermatozoa is simpler, less time-consuming, and less costly than of embryos for maintaining transgenic or gene-disrupted mouse strains, maintenance of cryopreserved spermatozoa still has high running costs because of the need for a constant supply of liquid nitrogen. It has been reported that freeze-dried mouse spermatozoa are capable of participating in normal embryonic development after injection into oocytes, and so they have attracted a great deal of attention as storable gene resources. However, it is particularly essential to assure long-term preservation for several decades or centuries. The application of the determination of accelerated degradation kinetics calculated by extrapolation of Arrhenius plots to the preservation of freeze-dried mouse spermatozoa is a possible solution. This theory also is being applied to long-term stability of drugs. In this issue, we introduce recent studies of freeze-dried mouse spermatozoa.<br>

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