ブタ精子抽出物の顕微注入によるブタ成熟卵母細胞の活性化とその後の発生
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- Okada Konosuke
- Department of Life Science, Graduate School of Science and Technology, Kobe University
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- Miyano Takashi
- Laboratory of Reproductive Biology and Biotechnology, Faculty of Agriculture, Kobe University
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- Miyake Masashi
- Department of Life Science, Graduate School of Science and Technology, Kobe University
書誌事項
- タイトル別名
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- Activation and Development of Pig Oocytes after Microinjection of Crude Sperm Extract
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説明
This work was undertaken to investigate the activation of pig oocytes after microinjection of crude sperm extract prepared from ejaculated boar spermatozoa. The results were compared with those of electro-stimulated oocytes. When in vitro-matured pig oocytes were microinjected with sperm extract and others were electro-stimulated, 100% and 92%, respectively, of the oocytes were released from arrest at metaphase II (MII) and formed female pronuclei. To test their developmental ability, the injected oocytes were treated with cytochalasin B and then cultured in NCSU23 medium. After 168 h, 30% and 44% of the oocytes that had been microinjected with sperm extract and electro-stimulated, respectively, developed to the blastocyst stage. At 6-8 h after the microinjection of sperm extract or electro-stimulation, cortical granules were released from the oocytes. In addition, Cdc2 kinase activity declined to a low level in the treated oocytes. These results indicate that microinjection of crude sperm extract induces the release of in vitro-matured pig oocytes from MII-arrest and leads them into a series of events related to oocyte activation.<br>
収録刊行物
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- 日本哺乳動物卵子学会誌
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日本哺乳動物卵子学会誌 21 (3), 134-140, 2004
日本卵子学会
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詳細情報 詳細情報について
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- CRID
- 1390282679315188608
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- NII論文ID
- 10014108967
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- NII書誌ID
- AN10548943
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- ISSN
- 13475878
- 13417738
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- NDL書誌ID
- 7173710
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
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