A Vitrification Method by Means of a Straw to Prevent Infections in Mouse Pronuclear Embryos

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Mouse pronuclear embryos were cryopreserved by a simple and safe vitrification method. In the process, Vitrification Media VT101, Thawing Media VT 102 (KITAZATO. Co. Japan) and the embryos were loaded into a straw; then they were cryopreserved. Different loading methods were examined to determine the safety levels of crystallization for the embryo's survival after thawing. The best condition attained, after thawing, was a 75% embryo survived rate of which 66% developed to the two-cell stage, 71% developed to the morula stage and 27% developed to the blastosyst stage. This development of embryos after vitrification was not significantly different to that of a control group without freezing and thawing. The vitrification method was considered to protect embryos against various infections via liquid nitrogen during cryopreservation. It is expected that the method can be applied to human embryos.<br>

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Details 詳細情報について

  • CRID
    1390282679315262208
  • NII Article ID
    10012455133
  • NII Book ID
    AN10548943
  • DOI
    10.1274/jmor.20.124
  • ISSN
    13475878
    13417738
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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