ICSIの際の卵細胞膜穿破様式と卵の質および受精,初期発生との関連

DOI 日本農学文献記事索引 Web Site 被引用文献1件 参考文献16件
  • 柳田 薫
    福島県立医科大学医学部産科学婦人科学講座
  • 片寄 治男
    福島県立医科大学医学部産科学婦人科学講座
  • 鈴木 和夫
    福島県立医科大学医学部産科学婦人科学講座
  • 近内 勝之
    福島県立医科大学医学部産科学婦人科学講座
  • 菅沼 亮太
    福島県立医科大学医学部産科学婦人科学講座
  • 佐藤 章
    福島県立医科大学医学部産科学婦人科学講座

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  • Flexibility of Oolemma is an Important Factor for Oocyte Survival after ICSI.

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We examined the relationship between the breakage modes occurring during intracytoplasmic sperm injection (ICSI) and survival, fertilization and embryonic development rates after ICSI, and the morphologic quality of the oocytes before ICSI. The tip of each injection pipette was flattened to exclude the factor of tip shape. Oolemma breakage modes were grouped into four types according to oolemma flexibility. Approximately 8% of the mature oocytes treated with ICSI featured an inflexible oolemma, and 50% of such oocytes resulted in cytolysis, but no significant differences were observed in fertilization rates among the surviving oocytes, and no relationship could be traced between breakage modes and morphologic oocyte quality. The selection of oocytes with flexible oolemma is believed to be crucial in obtaining satisfactory results with ICSI, but it is not yet possible to distinguish oocytes that have inflexible oolemma from other oocytes before ICSI.

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