体内または体外で発育したハムスター胚におけるミトコンドリアと細胞骨格の分布
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- Suzuki Hiroyuki
- Faculty of Agriculture and Life Science, Hirosaki University
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- Satoh Manabu
- Faculty of Agriculture and Life Science, Hirosaki University
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- Kabashima Katsuya
- Faculty of Agriculture and Life Science, Hirosaki University
書誌事項
- タイトル別名
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- Distributions of Mitochondria and the Cytoskeleton in Hamster Embryos Developed In Vivo and In Vitro
この論文をさがす
抄録
To clarify the causes of low viability of hamster embryos following in vitro culture, the present study compared the distributions of mitochondria and the cytoskeleton in embryos grown in vivo and in vitro. Hamster 2- and 4-cell embryos were characterized by perinuclear clustering of mitochondria, the degree of which was almost the same for in vivo and in vitro embryos. In the cell cortex and cell-to-cell contact region, however, microfilaments were located less densely in in vitro embryos than in in vivo ones. The nucleus moved towards the apex of the blastomere at the late 8-cell stage, when embryos begin the process of compaction. The density of mitochondria seemed to increase in the cell-to-cell contact region during this cellular rearrangement. Mitochondria were concentrated at the perinuclear region of in-vivo 8-cell embryos, whereas they were diffused into the subcortical region of in-vitro 8-cell embryos. Such a diffusion pattern of mitochondrial distribution was also noted in the morulae and blastocysts grown in vitro. These results show that both mitochondrial translocation and cytoskeletal reorganization did not proceed normally in the hamster embryos cultured in vitro, probably resulting in decreased viability of these embryos.<br>
収録刊行物
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- 日本哺乳動物卵子学会誌
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日本哺乳動物卵子学会誌 23 (3), 128-134, 2006
日本卵子学会
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詳細情報 詳細情報について
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- CRID
- 1390282679315436672
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- NII論文ID
- 10020355421
- 30024126760
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- NII書誌ID
- AN10548943
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- ISSN
- 13475878
- 13417738
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- NDL書誌ID
- 8628590
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可